拓世众和

      哺乳动物细胞/组织线粒体提取试剂盒由优化的缓冲液系统和2.0ml离心管柱组成。可以快速的从哺乳动物细胞/组织中提取线粒体蛋白。离心管柱技术使用方便简单，高产，与其他商业试剂盒相比，可以样品处理范围更广泛，可处理5-50million/样品，并且可以得到完整的线粒体。缓冲液系统不含表面活性剂和EDTA，无需匀浆和组织研磨，操作时间<30分钟。

原理：细胞/组织通过Buffer A致敏，然后细胞以Z字形路径通过离心管柱，在这个过程中细胞膜会破裂。进而通过差速离心和密度离心分离出完整的线粒体，整个过程无需匀浆和超高速离心。

提取出的线粒体可以应用于SDS-PAGE，immunoblottings,ELISA,IP,膜蛋白结构分析，2-D，酶活性检测和其他应用

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with MinuteTM Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.

简单，快速，性价比高

得率高，可获得更完整的线粒体

处理样品范围更广

缓冲液系统不含表面活性剂和EDTA

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